Detection of TBI is biggest challenge for medical science. Currently imaging techniques such as MRI, CT scan and x-ray are used to detect tbi but by using imaging techniques we can only detect the structural disruption in brain , it mean we can only detect the severe and moderate tbi. It’s impossible to detect the mild traumatic brain injury. There is urgent need for development of biomarkers for mild tbi. According to American government data, 3 million people get affected by mild traumatic brain injury. Most of them don’t know that they are suffering from mild tbi due to lack of diagnosis available for it.